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Professor
Federal University of Parana (UFPR), Brazil
Email: wandersondarocha@gmail.com
VALIDATE Role:
Network Affiliate
Research Keywords:
Trypanosoma cruzi, Leishmania, Attenuation, Virulence, Biotechnology
Biography:
The main goals of my research group are to develop genetically attenuated parasites (T. cruzi, and Leishmania) using genome editing tools, and identification and characterization of surface ligands to improve Chagas disease treatment. Attenuated parasites are being developed also to be used as vaccine vector.
Related Websites:
LinkedIn
UFPR Postgraduate Program In Sciences - Biochemistry
Key Publications:
- Saenz-Garcia JL, Borges BS, Souza-Melo N, Machado LV, Miranda JS, Pacheco-Lugo LA, Moretti NS, Wheleer R, Soares Medeiros LC, DaRocha WD. Trypanin Disruption Affects the Motility and Infectivity of the Protozoan Trypanosoma cruzi. Front Cell Infect Microbiol. 2022 Jan 7;11:807236. doi: 10.3389/fcimb.2021.807236. eCollection 2021.
- Pacheco-Lugo LA, Sáenz-García JL, Díaz-Olmos Y, Netto-Costa R, Brant RSC, DaRocha WD. CREditing: a tool for gene tuning in Trypanosoma cruzi. Int J Parasitol. 2020 Nov;50(13):1067-1077. doi: 10.1016/j.ijpara.2020.06.010. Epub 2020 Aug 25.
- Sáenz-Garcia JL, Yamanaka IB, Pacheco-Lugo LA, Miranda JS, Córneo ES, Machado-de-Ávila RA, De Moura JF, DaRocha WD. Targeting epimastigotes of Trypanosoma cruzi with a peptide isolated from a phage display random library. Exp Parasitol. 2020 Mar;210:107830. doi: 10.1016/j.exppara.2020.107830. Epub 2020 Jan 7.
- Demeu LMK, Soares RJ, Miranda JS, Pacheco-Lugo LA, Oliveira KG, Cortez Plaza CA, Billiald P, Ferreira de Moura J, Yoshida N, Alvarenga LM, DaRocha WD. Engineering a single-chain antibody against Trypanosoma cruzi metacyclic trypomastigotes to block cell invasion. PLoS One. 2019 Oct 16;14(10):e0223773. doi: 10.1371/journal.pone.0223773. eCollection 2019.
- Burle-Caldas GA, Soares-Simões M, Lemos-Pechnicki L, DaRocha WD, Teixeira SMR. Assessment of two CRISPR-Cas9 genome editing protocols for rapid generation of Trypanosoma cruzi gene knockout mutants. Int J Parasitol. 2018 Jul;48(8):591-596. doi: 10.1016/j.ijpara.2018.02.002. Epub 2018 Mar 22.
- de Paiva RM, Grazielle-Silva V, Cardoso MS, Nakagaki BN, Mendonça-Neto RP, Canavaci AM, Souza Melo N, Martinelli PM, Fernandes AP, daRocha WD, Teixeira SM. Amastin Knockdown in Leishmania braziliensis Affects Parasite-Macrophage Interaction and Results in Impaired Viability of Intracellular Amastigotes. PLoS Pathog. 2015 Dec 7;11(12):e1005296. doi: 10.1371/journal.ppat.1005296. eCollection 2015 Dec.
- Martins VT, Chávez-Fumagalli MA, Costa LE, Canavaci AM, Martins AM, Lage PS, Lage DP, Duarte MC, Valadares DG, Magalhães RD, Ribeiro TG, Nagem RA, Darocha WD, Régis WC, Soto M, Coelho EA, Fernandes AP, Tavares CA. Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis. PLoS Negl Trop Dis. 2013;7(3):e2148. doi: 10.1371/journal.pntd.0002148. Epub 2013 Mar 28.
- Junqueira C, Santos LI, Galvão-Filho B, Teixeira SM, Rodrigues FG, DaRocha WD, Chiari E, Jungbluth AA, Ritter G, Gnjatic S, Old LJ, Gazzinelli RT. Trypanosoma cruzi as an effective cancer antigen delivery vector. Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19695-700. doi: 10.1073/pnas.1110030108.
- Bartholomeu DC, Cerqueira GC, Leão AC, daRocha WD, Pais FS, Macedo C, Djikeng A, Teixeira SM, El-Sayed NM. Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi. Nucleic Acids Res. 2009 Jun;37(10):3407-17. doi: 10.1093/nar/gkp172. Epub 2009 Mar 31.
- DaRocha WD, Otsu K, Teixeira SM, Donelson JE. Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi. Mol Biochem Parasitol. 2004 Feb;133(2):175-86. doi: 10.1016/j.molbiopara.2003.10.005.
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