Publications of Interest to VALIDATE Members


Publications by VALIDATE members will be posted here (and these and further papers that may be of interest to VALIDATE members are posted on Twitter)

If you have, or spot, any relevant publications for VALIDATE members, please forward them to so that we can list them here.

Please could members ensure that any VALIDATE-related publications include the following acknowledgement: "This work was supported by the GCRF Networks in Vaccines Research and Development VALIDATE Network, which was co-funded by the MRC and BBSRC. This UK-funded award is part of the EDCTP2 programme supported by the European Union."


Revamping the BCG revaccination strategy: Records to recall, or just call it phoenix?, VALIDATE member Mario Alberto Flores-Valdez. In Human Vaccines & Immunotherapeutics. DOI: 10.1080/21645515.2023.2300158


BCG∆BCG1419c and BCG differ in induction of autophagy, c-di-GMP content, proteome, and progression of lung pathology in Mycobacterium tuberculosis HN878-infected male BALB/c mice, VALIDATE members Cristian Alfredo Segura-Cerda and Mario Alberto Flores-Valdez. In Vaccine. DOI 10.1016


Preprint: Persistent T cell unresponsiveness associated with chronic visceral leishmaniasis in HIV-coinfected patientsVALIDATE Members Nicky de Vrij, Thao-Thy Pham, and Wim Adriaensen et al. In Nature Portfolio. DOI 10.21203 


M. tuberculosis antigen-responsive IL17+ CD4 T cells are disproportionately spared in ART-suppressed HIVVALIDATE Member Paul Ogongo. In bioRx. DOI 10.1101


T cell receptor repertoires associated with control and disease progression following Mycobacterium tuberculosis infectionVALIDATE Member Munyaradzi Musvosvi et al. In Nature Medicine. DOI 10.21417.


Hcp1-loaded staphylococcal membrane vesicle vaccine protects against acute melioidosisVALIDATE member Nargis Khan et al. In Frontiers in Immunology. DOI 10.3389


Inflammation and immune activation are associated with risk of Mycobacterium tuberculosis infection in BCG-vaccinated infants. VALIDATE Members Iman Satti, Shuailin Li, Steph Harris, Rachel Tanner, Deniz Cizmeci, Helen Fletcher, and VALIDATENetwork Director Helen McShane. In Nature. DOI10.1038


RNA-seq reveals a type 6 secretion system-dependent blockage of TNF-α signaling and BicA as a Burkholderia pseudomallei virulence factor important during gastrointestinal infectionVALIDATE members Javier I. Sanchez-Villamil, Daniel Tapia et al. In Gut Microbes. DOI 10.1080


Mitochondrial Ultrastructure and Activity Are Differentially Regulated by Glycolysis-, Krebs Cycle-, and Microbiota-Derived Metabolites in Monocytes. C. Angélica Pérez-Hernández CA et al (including Project members Susanna Dunachie, Hazel M. Dockrell, Steven G. Smith and F. Javier Sánchez-García) In Biology. DOI 10.3390


Human Pulmonary Tuberculosis: Understanding the Immune Response in the Bronchoalveolar System Herrera TH et al including VALIDATE member Mario Alberto Flores-Valdez in Biomolecules


Towards Reverse Vaccinology for Bovine TB: High Throughput Expression of Full Length Recombinant Mycobacterium bovis Proteins, Frontiers in Molecular Biosciences. VALIDATE members Deepa Paliwal, Michelle Thom, and Ian M. Jones.


Evaluation of early innate and adaptive immune responses to the TB vaccine Mycobacterium bovis BCG and vaccine candidate BCGΔBCG1419cVALIDATE member Mario Alberto Flores-Valdez and Manuja Gunasena, Rajni Kant Shukla, Naiquan Yao, Oscar Rosas Mejia, Michael D. Powell, Kenneth J. Oestreich, Michel de Jesús Aceves-Sánchez,  Namal P. M. Liyanage & Richard T. Robinson.


Lower IL-7 Receptor Expression of Monocytes Impairs Antimycobacterial Effector Functions in Patients with Tuberculosis. Ernest Adankwah, Jean De Dieu Harelimana, Difery Minadzi, Wilfred Aniagyei, Mohammed K. Abass, Linda Batsa Debrah, Dorcas O. Owusu, Ertan Mayatepek, Richard O. Phillips and Marc Jacobsen. J Immunol May 15, 2021, 206 (10) 2430-2440; DOI:


ChimLeish, a new recombinant chimeric protein evaluated as a diagnostic and prognostic marker for visceral leishmaniasis and human immunodeficiency virus coinfection.Galvani NC, Machado AS, Lage DP, Freitas CS, Vale DL, de Oliveira D, Ludolf F, Ramos FF, Fernandes BB, Luiz GP, Mendonça DVC, Oliveira-da-Silva JA, Reis TAR, Tavares GSV, Chaves AT, Guimarães NS, Tupinambás U, Cota GF, Humbert MV, Martins VT, Christodoulides M, Coelho EAF, Machado-de-Ávila RA. Parasitol Res. 2021 Oct 19:1-11. doi: 10.1007/s00436-021-07342-1. Online ahead of print. PMID: 34664113


Potential of recombinant LiHyQ, a novel Leishmania infantum protein, for the diagnosis of canine visceral leishmaniasis and as a diagnostic and prognostic marker for human leishmaniasis and human immunodeficiency virus co-infection: A preliminary study. Santos TTO, Ramos FF, Gonçalves IAP, Tavares GSV, Ludolf F, Bandeira RS, Silva AM, Oliveira-da-Silva JA, Reis TAR, Machado AS, Lage DP, Freitas CS, Vale DL, Martins VT, Alves LA, Guimarães NS, Chaves AT, Chávez-Fumagalli MA, Cota GF, Silveira JAG, Tupinambás U, Gonçalves DU, Christodoulides M, Coelho EAF. Acta Trop. 2021 Dec;224:106126. doi: 10.1016/j.actatropica.2021.106126. Epub 2021 Sep 16. PMID: 34537185


In silico comparisons of lipid-related genes between Mycobacterium tuberculosis and BCG vaccine strains. Sarno A, Bitencourt J, Queiroz A, Arruda S. . Genet Mol Biol. 2021 Oct 22;44(4):e20210024. doi: 10.1590/1678-4685-GMB-2021-0024. PMID: 34699585; PMCID: PMC8547388.


TB and SIV Coinfection; a Model for Evaluating Vaccine Strategies against TB Reactivation in Asian Origin Cynomolgus Macaques: A Pilot Study Using BCG Vaccination. Vaccines (Basel). White AD, Sibley L, Gullick J, Sarfas C, Clark S, Fagrouch Z, Verschoor E, Salguero FJ, Dennis M, Sharpe S. 2021 Aug 25;9(9):945. doi: 10.3390/vaccines9090945. PMID: 34579182; PMCID: PMC8473354.


Balance between Protection and Pathogenic Response to Aerosol Challenge with Mycobacterium tuberculosis (Mtb) in Mice Vaccinated with TriFu64, a Fusion Consisting of Three Mtb Antigens, Mtb Antigens, Sadaf Sulman, Savidge BO, Alqaseer K, K. Das MK, Abadi ND,Pearl JE, Turapov O, Mukamolova GV, Akhtar W and Cooper AM. 2021 in MDPI


Increasing tuberculosis burden in Latin America: an alarming trend for global control efforts, T Ranzani T et al including VALIDATE member Alberto Garcia-Basteiro. 2021 in BMJ Global Health


Identification and phenotype of MAIT cells in cattle and their response to bacterial infections, Edmans MD et al including VALIDATE member Martin Vordermeier. 2021 in Frontiers in Immunology


Leishmaniasis immunopathology—impact on design and use of vaccines, diagnostics and drugs, VALIDATE member Paul M. Kaye et al 2021 in Seminars in Immunopathology


Antigen-specific antibody and polyfunctional T cells generated by respiratory immunization with protective Burkholderia ΔtonB Δhcp1 live attenuated vaccines, Khakhum N et al, including VALIDATE member Alfredo Torres. In NPJ Vaccines


Effects of different types of written vaccination information on COVID-19 vaccine hesitancy in the UK (OCEANS-III): a single-blind, parallel-group, randomised controlled trial, by Prof Daniel Freeman,   Bao Sheng Loe, Ly-Mee Yu, DPhil Jason Freeman, MA Prof Andrew Chadwick, Prof Cristian Vaccari, Milensu Shanyinde, Victoria Harris, Felicity Waite, Laina Rosebrock, Ariane Petit, Samantha Vanderslott, Prof Stephan Lewandowsky, Michael Larkin, Stefania Innocenti, Prof Andrew J Pollard, Prof Helen McShane, Sinéad Lambe. 2021 in The Lancet Public Health


Dissecting pathways to thrombocytopenia in a mouse model of visceral leishmaniasis, Rani GF et al including VALIDATE Member Paul Fine, in Blood Advances


Burkholderia pseudomallei as an enteric pathogen: identification of virulence factors mediating gastrointestinal infection, Javier Sanchez Villamil, Daniel Tapia, Alfredo Torres et al. 2020 American Society for Microbiology


Multinucleated giant cell formation as a portal to chronic bacterial infections, Jacob Stockton, Alfredo Torres. 2020 MDPI


Vaccination with intradermal Bacillus Calmette-Guérin provides robust protection against extrapulmonary tuberculosis but not pulmonary infection in cynomolgus macaques. TYusuke Tsujimura, Yumiko Shiogama, Shogo Soma, Tomotaka Okamura, Junichiro Takano, Emiko Urano, Yoshiko Murakata, Akira Kawano, Natsuko Yamakawa, Masamitsu N. Asaka, Kazuhiro Matsuo and Yasuhiro Yasutomi, 2020 Journal of Immunology


Potential impact of tuberculosis vaccines in China, South Africa, and India, Rebecca Harris, Tom Sumner, Gwenan M. Knight, Hui Zhang, Richard G White. 2020 Science Translational Medicine


Discovery and validation of a personalized risk predictor for incident tuberculosis in low transmission settings, , Rishi K. Gupta, Claire J. Calderwood, Alexei Yavlinsky, Maria Krutikov , Matteo Quartagno, Maximilian C. Aichelburg, Neus Altet, Roland Diel, Claudia C. Dobler , Jose Dominguez, Joseph S. Doyle, Connie Erkens, Steffen Geis, Pranabashis Haldar, Anja M. Hauri, Thomas Hermansen, James C. Johnston, Christoph Lange, Berit Lange, Frank van Leth, Laura Muñoz, Christine Roder, Kamila Romanowski, David Roth, Martina Sester, Rosa Sloot, Giovanni Sotgiu, Gerrit Woltmann, Takashi Yoshiyama, Jean-Pierre Zellweger, Dominik Zenner, Robert W. Aldridge, Andrew Copas, Molebogeng X. Rangaka, Marc Lipman, Mahdad Noursadeghi and Ibrahim Abubakar. 2020 Nature Medicine



Predicting toxins found in toxin–antitoxin systems with a role in host-induced Burkholderia pseudomallei persistence, Ross BN et al, including VALIDATE Member Alfredo Torres. 2020 Nature


Disease extent and anti‐tubercular treatment response correlates with Mycobacterium tuberculosis‐specific CD4 T‐cell phenotype regardless of HIV‐1 status, Catherine Riou, Elsa Du Bruyn, Sheena Ruzive, Rene T Goliath, Cecilia S Lindestam Arlehamn, Alessandro Sette, Alan Sher, Daniel L Barber, Rob Wilkinson. 2020 Clinical & Translational Immunology


Glycan–glycan interactions determine Leishmania attachment to the midgut of permissive sand fly vectors, Hall AR et al including VALIDATE Members Laith Yakob and Matthew Rogers. 2020 Chemical Science


Multinucleated giant cell formation as a portal to chronic bacterial infections, Jacob L Stockton and Alfredo Torres. 2020 Preprints


Mapping global trends in vaccine confidence and investigating barriers to vaccine uptake: a large-scale retrospective temporal modelling study, Heidi Larson and Emilie Karafillakis. 2020 The Lancet


Multicomponent gold nano-glycoconjugate as a highly immunogenic and protective platform against Burkholderia mallei, Daniel Tapia, Javier Sanchez-Villamil and Alfredo Torres. 2020 NJP Vaccines


Characterization of a new Leishmania major isolate for use in a controlled human infection model, Helen Ashwin, Jovana Sadlova, Barbora Vojtkova, Tomas Becvar, Patrick Lypaczewski, Eli Schwartz, Elizabeth Greensted, Katrien Van Bocxlaer, Marion Pasin, Kai S. Lipinski, Vivak Parkash, Greg Matlashewski, Alison M. Layton, Charles J. Lacey, Charles L. Jaffe, Petr Volf, Paul M. Kaye. 2020 preprint BioRxiv


Host urine immunological biomarkers as potential candidates for the diagnosis of tuberculosis, Osagie A. Eribo, Monkoe S. Leqheka, Stephanus T. Malherbe, Gian D. van der Spuy,  Gerhard Walzl, Novel N. Chegou. 2020 International Journal of Infectious Disease


BCG and BCGΔBCG1419c protect type 2 diabetic mice against tuberculosis via different participation of T and B lymphocytes, dendritic cells and pro-inflammatory cytokines, Segura-Cerda CA et al including VALIDATE Member Mario Alberto Flores-Valdez. 2020 NPJ Vaccines


Transcriptional portrait of M. bovis BCG during biofilm production shows genes differentially expressed during intercellular aggregation and substrate attachment, Mario Alberto Flores-Valdez et al. 2020 NPJ Vaccines


Dual host-intracellular parasite transcriptome of enucleated cells hosting Leishmania amazonensis: control of half-life of host cell transcripts by the parasite, C Orikaza, Hiro Goto and Eduardo Ramos Milton-Sanchez. 2020 Infection and Immunity


A new Leishmania hypothetical protein can be used for accurate serodiagnosis of canine and human visceral leishmaniasis and as a potential prognostic marker for human disease, AS Machado, Eduardo Coelho. 2020 Experimental Parasitology


Liposomal Formulation of ChimeraT, a Multiple T-Cell Epitope-Containing Recombinant Protein, Is a Candidate Vaccine for Human Visceral Leishmaniasis, Lag DP et al including VALIDATE Members Vivian Tamietti MartinsEduardo Coelho and Myron Christodoulides, in MDPI


Identification of gene fusion events in Mycobacterium tuberculosis that encode chimeric proteins,  VALIDATE Member Jomien Mouton et al, in NAR Genomics and Bioinformatics


Genetic screening for the protective antigenic targets of BCG vaccination, A Smith, Bernardo Villarreal-RamosJohnjoe Mcfadden and Martin Vordermeier. 2020 Tuberculosis


The potential impact of human visceral leishmaniasis vaccines on population incidence, Epke A. Le Rutte, Luc E. Coffeng, Stefano Malvolti, Paul Kaye, Sake J. de Vlas. 2020 Plos NTD


Mitochondrial signature in human monocytes and resistance to infection in C. elegans during fumarate-induced Innate immune training, AC Pérez-Hernández,  Steven SmithHazel DockrellEgle Butkeviciute, and Francisco Javier Sánchez-Garcia. 2020 Frontiers in Immunology.


Recent Developments in the Application of Flow Cytometry to Advance our Understanding of Mycobacterium tuberculosis Physiology and Pathogenesis, Jomien Mouton et al. 2020 Cytometry


Preclinical assessment of a new live attenuated Mycobacterium tuberculosis Beijing based vaccine for tuberculosis, F Levillain  Ann Rawkins, Greg Bancroft and Olivier Neyrolles. 2020 Vaccine


Melioidosis in Gujarat: a neglected tropical disease, Tushar Shaw et al. 2020 Tropical Doctor


Mycobacterium tuberculosis cords within lymphatic endothelial cells to evade host immunity, Thomas R. Lerner, Christophe J. Queval, Rachel P. Lai, Matthew R.G. Russell, Antony Fearns, Daniel J. Greenwood, Lucy Collinson, Robert J. Wilkinson, Maximiliano G. Gutierrez. 2020 JCI Insight


Design of polymeric nanocapsules for intranasal vaccination against Mycobacterium tuberculosis: influence of the polymeric shell and antigen positioning, Lara Diego-González, José Crecente-Campo, OrcID, Matthew John Paul, Mahavir Singh, Rajko Reljic, María José Alonso, África González-Fernández, Rosana Simón-Vázquez. 2020 Pharmaceutics


The diversity putbred mouse population is an improved animal model of vaccination against tuberculosis that reflects heterogeneity of protection - Sherry L. Kurtz, Amy P. Rossi, Gillian L. Beamer, Dan M. Gatti, Igor Kramnik, Karen L. Elkins. 2020 mSphere


Melioidosis In Suspected Recurrent Tuberculosis: A disease in disguise, authors include VALIDATE members Tushar Shaw and Chiranjay Mukhopadhyay. 2020 JIDC


Protecting the world: Celebrating 200 years of UK vaccine research. 2020 British Society for Immunology


New live attenuated tuberculosis vaccine MTBVAC induces trained immunity and confers protection against experimental lethal pneumonia. Raquel Tarancón, Jorge Domínguez-Andrés, Santiago Uranga, Anaísa V.Ferreira, Laszlo A. Groh, Mirian Domenech, Fernando González-Camacho, Niels P.Riksen, Nacho Aguilo, José Yuste, Carlos Martín, Mihai G. Netea et al. 2020 PLoS Pathog


Melioidosis In Suspected Recurrent Tuberculosis: A disease in disguise, authors include VALIDATE members Tushar Shaw and Chiranjay Mukhopadhyay, 2020 JIDC


BCG as a Case Study for Precision Vaccine Development: Lessons From Vaccine Heterogeneity, Trained Immunity, and Immune Ontogeny. 2020 Frontiers in Microbiology


Ecology of Sand Flies: (Psychodidae: Phlebotominae) Natural Infection of Pintomyia townsendi With Leishmania amazonensis in a Cutaneous Leishmaniasis Focus in Colombia. 2020 Journal of Medical Entymology


Altered Proteome Of A Burkholderia Pseudomallei Mutant Defective in Short-Chain Dehydrogenase Affects Cell Adhesion, Biofilm Formation And Heat Stress Tolerance. by VALIDATE Member Onrapak Reamtong, Nitaya Indrawattana, Amporn Rungruengkitkun, Tipparat Thiangtrongjit, Taksaon Duangurai, Manas Chongsa-Nguan, Pornpan Pumirat, 2020 in Peerj


Adaption of the ex vivo mycobacterial growth inhibition assay for use with murine lung cells, Hannah Painter, Satria A. Prabowo, Felipe Cia, Lisa StockdaleRachel Tanner, Samuel Willcocks, Rajko ReljicHelen A. FletcherAndrea Zelmer. 2020 Scientific Reports


Human humoral immune responses to burkholderia pseudomallei

Human Immune Responses to Melioidosis and Cross-Reactivity to Low-Virulence Burkholderia Species, ThailandRongkard PKronsteiner B, Hantrakun V, Jenjaroen K, Sumonwiriya M, Chaichana P, Chumseng S, Chantratita N, Wuthiekanun V, Fletcher H, Teparrukkul P, Limmathurotsakul D, Day NPJ, Dunachie S. 2020 Emerging Infectious Diseases


Towards new TB vaccines. Benedict Brazie, Helen McShane. 2020 Seminars in Immunopathology


A phase 1 antigen dose escalation trial to evaluate safety, tolerability and immunogenicity of the leprosy vaccine candidate LepVax (LEP-F1 + GLA-SE) in healthy adults Duthie MS, Frevol A, Day T, Coler R, Vergara J, Rolf T, Sagawa ZK, Marie Beckmann A, Casper C, Reed SG. 2019 Vaccines


A phase I trial evaluating the safety and immunogenicity of a candidate tuberculosis vaccination regimen, ChAdOx1 85A prime – MVA85A boost in healthy UK adults Morven Wilkie, Iman Satti, Alice Minhinnick, Stephanie Harris, Michael Riste, Raquel Lopez Ramon, Sharon Sheehan, Zita-Rose Manjaly Thomas, Daniel Wright, Lisa Stockdale, Ali Hamidi, Matthew K. O'Shea, Kritica Dwivedi, Hannah Michaela Behrens, Tamara Davenne, Joshua Morton, Samantha Vermaak, Alison Lawrie, Paul Moss, Helen McShane. 2020 Vaccine


Peptide identification and selection of vaccine candidates

Identification of antigens presented by MHC for vaccines against tuberculosis. Paulo Bettencourt, Julius Müller, Annalisa Nicastri, Daire Cantillon, Meera Madhavan, Philip D. Charles, Carine B. Fotso, Rachel Wittenberg, Naomi C. Bull, Nawamin PinpathomratSimon J. WaddellElena Stylianou, Adrian V. S. Hill, Nicola Ternette, Helen McShane. 2020 npj Vaccines 


Tools for assessing the protective efficacy of TB vaccines in humans: in vitro mycobacterial growth inhibition predicts outcome of in vivo mycobacterial infection. Rachel TannerIman SattiStephanie Harris, Matthew K. O'Shea, Deniz Cizmeci, Daniel O'Connor, Agnieszka Chomka, Magali Matsumiya, Rachel Wittenberg, Angela M. Minassian, Joel Meyer, Helen A. Fletcher, Helen McShane. 2020 Frontiers in Immunology


Conceptual framework depicts several pathways through which social protection interventions may affect TB outcomes

A systematic review of the impact of psychosocial factors on immunity: Implications for enhancing BCG response against tuberculosis. Sally E. Hayward, Jennifer B. Dowd, Helen A. Fletcher, Laura B. Nellums, Fatima Wurie, Delia Boccia. 2020 SSM - Population Health


Cytomegalovirus infection is a risk factor for tuberculosis disease in infants. By VALIDATE members Rachel Tanner, Iman Satti, Stephanie Harris, Lisa Stockdale, Mbandi Kimbung, Helen McShane, Helen Fletcher and collaborators. 2019 JCL Insight


Mucosal delivery of tuberculosis vaccines: a review of current approaches and challengesElena Stylianou, Matthew J. Paul, Rajko ReljicHelen McShane. 2019 Expert Review of Vaccines.


Development of a diagnostic compatible BCG vaccine against Bovine tuberculosis. Chandran A, Williams K, Mendum T, Stewart G, Clark S, Zadi S, McLeod N, Williams AVillarreal-Ramos BVordermeier M, Maroudam V, Prasad A, Bharti N, Banerjee R, Manjari Kasibhatla S, McFadden J. 2019 Scientific Reports


Controlled human infection models - is it really feasible to give people tuberculosis? Helen McShane. 2019 American Journal of Respiratory and Critical Care Medicine


Final analysis of a trial of M72/ASO1E vaccine to prevent tuberculosis. Dereck R. Tait, Mark Hatherill, Olivier Van Der Meeren, Ann M. Ginsberg, Elana Van Brakel, Bruno Salaun, Thomas J. Scriba, Elaine J. Akite, Helen M. Ayles, Anne Bollaerts, Marie-Ange Demoitié, Andreas Diacon, Thomas G. Evans, Monde MuyoyetaRobert J. Wilkinson, et al. 2019 The New England Journal of Medicine


Clinical, microbiologic, and immunologic determinants of mortality in hospitalized patients with HIV-associated tuberculosis: A prospective cohort study. Charlotte Schutz, David Barr, Bruno B. Andrade, Muki Shey, Amy Ward, Saskia Janssen, Rosie Burton, Katalin A. Wilkinson, Bianca Sossen, Kiyoshi F. Fukutani, Mark Nicol, Gary Maartens, Robert J. Wilkinson, Graeme Meintjes. 2019 PLoS Medicine


Schematic representation of some of the proposed mechanisms of Ab action agains MTB infection

Emerging themes for the role of antibodies in tuberculosis. Andy C. Tran, Mi-Young Kim, Rajko Reljic. 2019 Immune Network


Insights and challenges in tuberculosis vaccine development. Helen McShane. 2019 The Lancet Respiratory Medicine


Live-attenuated Mycobacterium tuberculosis vaccine MTBVAC versus BCG in adults and neonates: a randomised controlled, double-blind dose-escalation trial. Michele Tameris, Helen Mearns, Adam Penn-Nicholson, Yolande GreggNicole BilekSimbarashe MabweHennie Geldenhuys, Justin ShenjeAngelique LuabeyaIngrid MurilloJuana Doce, Nacho Aguilo, Dessislava Marinova, Eugenia Puentes, Esteban Rodríguez, Jesús Gonzalo-AsensioBernard FritzellJelle TholeCarlos Martin, Thomas J. Scriba, Mark Hatherill. 2019 The Lancet Respiratory Medicine


The global impact and cost-effectiveness of a melioidosis vaccine. Nantasit Luangasanatip, Stefan Flasche, David A. B. Dance, Direk Limmathurotsakul, Bart J. Currie, Chiranjay Mukhopadhyay, Tim Atkins, Richard Titball, Mark Jit. 2019 BMC Medicine


Changes in transcript, metabolite, and antibody reactivity during the early protective immune response in humans to Mycobacterium tuberculosis infection. January Weiner, Teresa Domaszewska, Simon Donkor, Stefan H. E. Kaufmann, Philip C. Hill, Jayne S. Sutherland. 2019 Clinical Infectious Diseases


Immunometabolic signatures predict risk of progression to active tuberculosis and disease outcome. Fergal J. Duffy, January Weiner, Scott Hansen, David L. Tabb, Sara Suliman, Ethan Thompson, Jeroen Maertzdorf, Smitha Shankar, Gerard Tromp, Shreemanta Parida, Drew Dover, Michael K. Axthelm, Jayne S. Sutherland, Hazel M. Dockrell, Tom H. M. Ottenhoff, Thomas J. Scriba, Louis J. Picker, Gerhard Walzl, Daniel E. Zak and The GC6-74 Consortium. 2019 Frontiers in Immunology


Comparison of genetically distinct populations of macaques in terms of control of TB disease

Differences in monocyte: lymphocyte ratio and tuberculosis disease progression in genetically distinct populations of macaques. Laura Sibley, Karen Gooch, Alice Wareham, Susan Gray, Andrew Chancellor, Stuart Dowall, Simon Bate, Anthony Marriott, Mike J. Dennis, Andrew D. White, Philip D. Marsh, Helen A. Fletcher, Sally Sharpe. 2019 Scientific Reports


The humoral immune response to BCG vaccination. Rachel Tanner, Bernardo Villarreal-Ramos, Martin Vordermeier, Helen McShane. 2019 Frontiers in Immunology


Survival of Mycobacterium bovis BCG oral vaccine during transit through a dynamic in vitro model simulating the upper gastrointestinal tract of badgers. Gareth A. Williams, Marjorie E. Koenen, Robert Havenaar, Paul Wheeler, Sonya Gowtage, Sandrine Lesellier, Mark A. Chambers. 2019 PloS ONE


The way forward for tuberculosis vaccines. Richard G. White, Willem A. Hanekom, Johan Vekemans, Rebecca C. Harris. 2019 The Lancet Respiratory Medicine


Immune response and growth inhibition following historical BCG vaccination

Historical BCG vaccination combined with drug treatment enhances inhibition of mycobacterial growth ex vivo in human peripheral blood cells. Satria A. Prabowo, Andrea ZelmerLisa Stockdale, Utkarsh Ojha, Steven G. SmithKarin Seifert, Helen A. Fletcher. 2019 Scientific Reports


HIV, HCMV and mycobacterial antibody levels: a cross-secontioal study in a rural Ugandan cohort. Lisa Stockdale, Stephen Nash, Angela Nalwoga, Lorna Gibson, Hannah Painter, John G. Raynes, Gershim Asiki, Robert Newton, Helen A. Fletcher. 2019 Tropical Medicine & International Health


Optimisation, harmonisation and standardisation of the direct mycobacterial growth inhibition assay using cryopreserved human periphera blood mononuclear cells. Rachel Tanner, Steven G. Smith, Krista E. van Meijgaarden, Federico Giannoni, Morven Wilkie, Lucia Gabriele, Carla Palma, Hazel M. Dockrell, Tom H. M. Ottenhoff, Helen McShane. 2019 Journal of Immunological Methods


Study design diagram and monitoring of TB infecvtion by IFNy release after repeated ultra-low dose expose to M.tb

Special Issue book "Global Burden and Challenges of Melioidosis". Eds Direk Limmathurotsakul, David A. B. Dance Mar 2019


Prevention of tuberculosis infection and disease by local BCG in repeatedly exposed rhesus macaques. Karin Dijkman, Claudia C. Sombroek, Richard A. W. Vervenne, Sam O. Hofman, Charelle Boot, Edmond J. Remarque, Clemens H. M. Kocken, Tom H. M. Ottenhoff, Ivanela Kondova, Mohammed A. Khayum et al. 2019 Nature Medicine 


Dose finding for new vaccines: The role for immunostimulation/ immunodynamic modelling. Sophie J. Rhodes, Gwenan M. Knight, Denise E. Kirschnerb, Richard G. White, Thomas G. Evans. 2019 J Theoretical Biology


DNA plasmid coding for Phlebotomus sergenti salivary protein PsSP9, a member of the SP15 family of proteins, protects against Leishmania tropica. Elham Gholami, Fabiano Oliveira, Tahereh Taheri, Negar Seyed, Safoora Gharibzadeh, Nasim Gholami, et al. 2019 PLOS Neglected Tropical Diseases



lppz induces high cellular responsiveness in tb patients

Mycobacterial Lipoprotein Z Triggers Efficient Innate and Adaptive Immunity for Protection Against Mycobacterium tuberculosis Infection. Chen Yingying, Xiao Jia-ni, Li Yong, Xiao Yang-jiong, Xiong Yan-qing, Liu Ying, Wang Shu-jun, Ji Ping, Zhao Guo-ping, et al. 2019 Frontiers in Immunology


Elevated circulating levels of monocyte activation markers among tuberculosis patients with diabetes co‐morbidity. Nathella P. Kumar, Kadar Moideen, Yukthi Bhootra, Arul Nancy, Vijay Viswanathan, Basavaradhya S.  Shruthi, Shanmugam Sivakumar, Mohan Natarajan, Hardy Kornfeld, and Subash Babu. 2018 Immunology.


Clinical utility of existing and second-generation interferon-γ release assays for diagnostic evaluation of tuberculosis: an observational cohort study. Hilary S. Whitworth, Amarjit Badhan, Aime A. Boakye, Yemisi Takwoingi, Melanie Rees-Roberts,Christopher Partlett, Heather Lambie, John Innes, Graham Cooke, et al. 2019 Lancet Infectious Diseases.

gating strategy for analysis of multifunctional t cells

Mycobacterium leprae Recombinant Antigen Induces High Expression of Multifunction T Lymphocytes and Is Promising as a Specific Vaccine for Leprosy. Márcio Bezerra-Santos, Marise do Vale-Simon, Aline Silva Barreto, Rodrigo Anselmo Cazzaniga, Daniela Teles de Oliveira, Mônica Rueda Barrios, Alex Ricardo Ferreira, Nanci Santos-Bio, Steven G. Reed, et al. 2018 Frontiers in Immunology


Next-generation Diagnostics for Melioidosis: Evaluation of a Prototype i-STAT Cartridge to Detect Burkholderia pseudomallei Biomarkers. ​​​​​​ Kevin L. Schully, Charles C. Young, Mark Mayo, Amy L. Connolly, Vanessa Rigas, Ammarah Spall, Alyssa A. Chan, Mark G. Salvador, James V. Lawler, et al. 2018 Clinical Infectious Diseases


Age-targeted tuberculosis vaccination in China and implications for vaccine development: a modelling study. Rebecca C. Harris, Tom Sumner, Gwenan M. Knight, Thomas G. Evans, Vicky Cardenas, Chen Chen, Richard G. White. 2019 The Lancet Global Health


schematic representation of tyk2 dependent signaling pathways

Tuberculosis and impaired IL-23–dependent IFN-γ immunity in humans homozygous for a common TYK2missense variant. Stéphanie Boisson-Dupuis, Noe Ramirez-Alejo, Zhi Li, Etienne Patin, Geetha Rao, Gaspard Kerner, Che Kang Lim, Dimitry N. Krementsov, Nicholas Hernandez, et al. 2018 Science Immunology


Human IFN-γ immunity to mycobacteria is governed by both IL-12 and IL-23. Rubén Martínez-Barricarte, Janet Markle, Cindy Ma, Elissa Deenick, Noé Ramírez-Alejo, Federico Mele, Daniela Latorre, Seyed Alireza Mahdaviani, Caner Aytekin et al. 2018 Science Immunology


Current approaches toward identifying a correlate of immune protection from tuberculosis. Iman Satti, Helen McShane. 2019 Expert Review of Vaccines


 sufrtb fails to grow under a low iron condition

Iron homeostasis in Mycobacterium tuberculosis is essential for persistence. Manitosh Pandey, Sakshi Talwar, Sutapa Bose, Amit Kumar Pandey. 2018 Scientific Reports


Burkholderia pseudomallei ΔtonB Δhcp1 Live Attenuated Vaccine Strain Elicits Full Protective Immunity against Aerosolized Melioidosis Infection. Nittaya Khakhum, Preeti Bharaj, Julia N. Myers, Daniel Tapia, Paul B. Kilgore, Brittany N. Ross, David H. Walker, Janice J. Endsley, Alfredo G. Torres. 2018 mSphere


Regulation of mycobacterial infection by macrophage Gch1 and tetrahydrobiopterin. Eileen McNeill, Elena Stylianou, Mark J. Crabtree, Rachel Harrington-Kandt, Anna-Lena Kolb, Marina Diotallevi, Ashley B. Hale, Paulo Bettencourt, Rachel Tanner, Matthew K. O’Shea, Magali Matsumiya, Helen Lockstone, Julius Müller, Helen A. Fletcher, David Greaves, Helen McShane, Keith Channon. 2018 Nature Communications


Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains. Jéssica da Silva Ferreira, Diego Augusto Souza, João Pedro Santos, Carla Carolina Dias Uzedo Ribeiro, Bruna A. Baêta, Rafaella Câmara Teixeira, Arthur da Silva Neumann, Patricia Sammarco Rosa, Maria Cristina Vidal Pessolani, et al. 2018 PLOS Neglected Tropical Diseases


Human Hookworm Infection Enhances Mycobacterial Growth Inhibition and Associates With Reduced Risk of Tuberculosis Infection. Matthew K. O’Shea, Thomas E. Fletcher, Julius Muller, Rachel Tanner, Magali Matsumiya, Jennifer W. Bailey, Jayne Jones, Steven G. Smith, Gavin Koh, William G. Horsnell, Nicholas J. Beeching, James Dunbar, Duncan Wilson , Adam F. Cunningham and Helen McShane. 2018 Frontiers in Immunology



Validation of a new serological assay for the identification of Mycobacterium tuberculosis complex-specific antibodies in pigs and wild boar. Jobin Thomas, Jose A. Infantes-Lorenzo, Inmaculada Moreno, David Cano-Terriza, Lucía de Juan, Ignacio García-Bocanegra, Lucas Domínguez, Mercedes Domínguez, Christian Gortázar, and María A. Risalde. 2019 Preventative Veterinary Medicine


Vaccine Hesitancy: Towards a Better Understanding of Drivers and Barriers to Awareness, Acceptance and Activation. Special Edition. 2018 Vaccine


Age-related warning of immune responses to BCG in healthy children

Age-related waning of immune responses to BCG in healthy children supports the need for a booster dose of BCG in TB endemic countries. Elizabeth Whittaker, Mark P. Nicol, Heather J. Zar, Nontobeko G. Tena-Coki, Beate Kampmann. 2018 Scientific Reports


Characterising antibody avidity in individuals of varied Mycobacterium tuberculosis infection status using surface plasmon resonance. Simon G. Kimuda, Irene Andia Biraro, Bernard S. Bagaya, John G. Raynes, Stephen Cose. 2018 PLOS One


Vaccines for Leprosy and Tuberculosis: Opportunities for Shared Research, Development, and Application. Mariateresa Coppola, Susan J. F. van den Eeden, Naoko Robbins, Louis Wilson, Kees L. M. C. Franken, Linda B. Adams, Tom P. Gillis, Tom H. M. Ottenhoff, Annemieke Geluk. 2018 Frontiers in Immunology


Tissue and host species-specific transcriptional changes in models of experimental visceral leishmaniasis. Helen Ashwin, Karin Seifert, Sarah Forrester, Najmeeyah Brown, Sandy MacDonald, Sally James, Dimitris Lagos, Jon Timmis, Jeremy Mottram, Simon L. Croft, Paul M. Kaye. 2018 Wellcome Open Research


TBVAC2020: Advancing Tuberculosis Vaccines from Discovery to Clinical Development. Stefan H. E. Kaufmann, Hazel M. Dockrell, Nick Drager, Mei Mei Ho, Helen McShane, Olivier Neyrolles, Tom H. M. Ottenhoff, Brij Patel, Danielle Roordink, François Spertini, Steffen Stenger, Jelle Thole, Frank Verreck, Ann Williams, TBVAC2020 Consortium. 2018 Frontiers in Immunology


Glibenclamide reduces primary human monocyte functions against tuberculosis infection


Glibenclamide Reduces Primary Human Monocyte Functions Against Tuberculosis Infection by Enhancing M2 PolarizationChidchamai Kewcharoenwong, Satria A. Prabowo, Gregory Bancroft, Helen A. FletcherGanjana Lertmemongkolchai. 2018 Frontiers in Immunology


Special Issue: Global Burden and Challenges of Melioidosis - 2018 Tropical Medicine and Infectious Disease. Including 22 Published Papers


Speeding Access to Vaccines and Medicines in Low- and Middle-Income Countries: A Case for Change and a Framework for Optimized Product Market Authorization. Vincent Ahonkai, Samuel Martins, Alexandre Portet, Murray Lumpkin, Dan Hartman. 2018 PLOS One


Phase 2b Controlled Trial of M72/AS01E Vaccine to Prevent Tuberculosis. Olivier Van Der Meeren, Mark Hatherill, Videlis Nduba, Robert J. WilkinsonMonde Muyoyeta, Elana Van Brakel, Helen M. Ayles, German Henostroza, Friedrich Thienemann, Thomas J. Scriba, Andreas Diacon, Gretta L. Blatner, Thomas G. Evans, et al. 2018 New England Journal of Medicine


Lessons from the first clinical trial of a non-licensed vaccine among Ugandan adolescents: a phase II field trial of the tuberculosis candidate vaccine, MVA85A. Anne Wajja, Milly Namutebi, Barbara Apule, Gloria Oduru, Samuel Kiwanuka, Mirriam AkelloBeatrice Nassanga, Joyce Kabagenyi, Juma Mpiima, Samantha Vermaak, Alison Lawrie, Iman Satti, Jaco Verweij, Stephen Cose, Jonathan Levin, Pontiano Kaleebu, Edridah Tukahebwa, Helen McShaneAlison Elliott. 2018 Wellcome Open Research


ifn y responses

Using vaccine Immunostimulation/Immunodynamic modelling methods to inform vaccine dose decision-making. Sophie J. Rhodes, Jeremie Guedj, Helen A. Fletcher, Thomas Lindenstrøm, Thomas J. Scriba, Thomas G. Evans, Gwenan M. Knight. Richard G. White. 2018 npj Vaccines


Safety and immunogenicity of the novel tuberculosis vaccine ID93 + GLA-SE in BCG-vaccinated healthy adults in South Africa: a randomised, double-blind, placebo-controlled phase 1 trial. Adam Penn-Nicholson, Michele Tameris, Erica Smit, Tracey Day, Munyaradzi Musvosvi, Lakshmi Jayashankar, et al. 2018 The Lancet Respiratory Medicine


Heterologous effects of infant BCG vaccination: potential mechanisms of immunity. Egle Butkeviciute, Christine E. Jones, Steven G. Smith. 2018 Future Microbiology



Leishmaniasis. Sakib Burza, Simon L. Croft, Marleen Boelaert. 2018 The Lancet


Isolation and characterisation of alveolar type II pneumocytes from adult bovine lung. Diane Frances LeeFrancisco Javier Salguero, Duncan Grainger, Robert James Francis, Kirsty MacLellan-Gibson, Mark A. Chambers. 2018 Scientific Reports


Induction and maintenance of a phenotypically heterogeneous lung tissue-resident CD4+ T cell population following BCG immunisationNaomi C. Bull, Daryan A. Kaveh, Carmen Garcia-Pelayo, Elena Stylianou, Helen McShane, Philip J. Hogarth. 2018 Vaccine


Melioidosis in Thailand: Present and FutureSoawapak Hinjoy et al (VALIDATE members: Ganjana Lertmemongkolchai, Narisara Chantratita, Apichai Taunyok, Susanna Dunachie, Surasakdi Wongratanacheewin). 2018 Trop Med Infect Dis [read other 'Melioidosis in...' papers in this Melioidosis Special Issue]


VALIDATE F1000 publication

VALIDATE: Exploiting the synergy between complex intracellular pathogens to expedite vaccine research and development for tuberculosis, leishmaniasis, melioidosis and leprosy. Helen A. Fletcher, Mitali Chatterjee, Andrea Cooper, Tracy Hussell, Paul M. Kaye, Joann Prior, Rajko Reljic, Samantha Vermaak, Martin Vordermeier, Ann Williams, Helen McShane. 2018 F1000Research


Factors influencing the higher incidence of tuberculosis among migrants and ethnic minorities in the UK. Sally E. Hayward, Rosalind M. Harding, Helen McShane, Rachel Tanner. 2018 F1000Research


Social determinants and BCG efficacy: a call for a socio-biological approach to TB prevention. Jennifer B. Dowd, Helen A. Fletcher, Delia Boccia. 2018 F1000Research


Melioidosis. W. Joost Wiersinga, Harjeet S. Virk, Alfredo G. Torres, Bart J. Currie, Sharon J. Peacock, David A. B. Dance, Direk Limmathurotsakul. 2018 Nature Reviews Disease Primers


Global Burden & Challenges of Melioidosis. David A. B. Dance, Direk Limmathurotsakul. 2018 Tropical Medicine and Infectious Disease


Development of a non-human primate BCG infection model for the evaluation of candidate tuberculosis vaccines. Stephanie A. Harris, Andrew D. White, Lisa Stockdale, Rachel Tanner, Laura Sibley, Charlotte Sarfas, Joel Meyer, Jonathan Peter, Matthew K. O'Shea, Zita-Rose Manjaly Thomas, Ali Hamidi, Iman Satti, Mike J. Dennis, Helen McShane, Sally Sharpe. 2018 Tuberculosis


Infection with Burkholderia pseudomallei - immune correlates of survival in acute melioidosis

Infection with Burkholderia pseudomallei - immune correlates of survival in acute melioidosis. Susanna J Dunachie, Kemajittra Jenjaroen, Catherine J. Reynolds, Kathryn J. Quigley, Ruhena Sergeant, Manutsanun Sumonwiriya, Panjaporn Chaichana, Suchintana Chumseng, Pitchayanant Ariyaprasert, Patricia Lassaux, Louise Gourlay, Charuporn Promwong, Prapit Teparrukkul, Direk Limmathurotsakul, Nicholas P. J. Day, Daniel M. Altmann, Rosemary J. Boyton. 2017 Scientific Reports